CTLA-4:B7-1 Inhibitor Screening Assay Kit
The CTLA-4/B7-1 binding assay kit is a TR-FRET based assay, which is designed to detect the binding status between CTLA-4 and B7-1. Tag6-CTLA-4 and Tag7-B7-1 are included in this assay kit. Binding of Tag6-CTLA-4 to Tag7-B7-1 brings the Terbium (Tb, HTRF donor) and the fluorophore d2 (HTRF acceptor) in a proximity distance, and activation of Tb results in fluorescence resonance energy transfer (FRET). Thus, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620 nm). Interference of the CTLA-4/B7-1 binding will reduce the HTRF signal.
