PARP2 (Poly (ADP-ribose) polymerase 2) is a member of the PARP family and plays a crucial role in DNA repair, particularly in the repair of single-strand breaks (SSBs) in DNA. It binds to DNA at the site of damage, becomes catalytically activated, and uses NAD⁺ as a substrate to add poly (ADP-ribose) (PAR) chains to itself and other proteins—a process called PARylation that results in the recruitment of other DNA repair proteins to the damaged site. Because of the high negative charge of PAR polymers, extensive autoPARylation of PARP2 leads to the dissociation of PARP2 from DNA, which is required for DNA repair completion. PARP2 is often overexpressed in various cancers, including breast, ovarian, prostate, lung, and glioblastoma. This overexpression is thought to support tumor cell survival. Some PARP inhibitors not only block the catalytic activity of PARP2 but also trap PARP2 on DNA at sites of damage, preventing its release. This creates a toxic DNA-protein complex that interferes with DNA replication and repair, leading to cell death, particularly in cancer cells deficient in homologous recombination repair (e.g., BRCA1/2-mutant cells).

TR-FRET PARP2 Trapping Assay Kit Information