OX40/OX40L Inhibitor Binding Assay Kit
Our OX40-OX40L binding assay kit is a TR-FRET based assay designed to detect the binding status between OX40 and OX40L. Tag5-OX40 and Tag7-OX40L are included in this assay kit. Binding of Tag5-OX40 to Tag7-OX40L brings the Terbium (Tb, HTRF donor) and the fluorophore d2 (HTRF acceptor) into proximity, and activation of Tb results in fluorescence resonance energy transfer (FRET). Therefore, the binding status can be quantitively measured by calculating the ratio of the emission fluorescence intensity of the acceptor (665 nm) and donor (620 nm). Disruption of OX40-OX40L binding will reduce the HTRF signal.
